geneclone相关论文
Cloning and Expression pattern of a Cuticular Protein Gene(CP2-130)in Antheraea pernyi during embryo
Bivoltine tussah silkworm variety “Xuan Da No.1” was used to investigate the changes in embryo development in Antherae......
Grain chalkiness greatly affects the grain appearance and milling, eating,cooking, and nutritional qualities, so it is o......
Objective: To study the effects of catalase (CAT) overexpression on reactive oxygen species level and phenotype of malig......
Molecular cloning and expression analyses of RPS3aGene from mulberry under abiotic stresses and amon
A full-length cDNA sequence coding ribosomal protein S3a of mulberry tree,which we designated MmRPS3a(GenBank accessionn......
Expression analysis of microtubule-associated gene HO19 in Dendrobium chrysanthumthum pistils upon s
Background: Plant self-incompatibility(SI)is called self-sterile plants,SI is more common in flowering monoecious plants......
HPLC and LC-TOF/MS were applied to determine theanine in the roots and leaves in the young and mature Dabieshan wild Cam......
利用质粒pAT153为载体,构建了莱姆螺旋体全细胞DNA基因文库,用菌落原位固相酶斑法从文库中初选出一株表达60kD抗原的克隆子,命名为pLW227.经免疫印迹分析,核......
【目的】钙结合蛋白作为钙信号传导途径的组分,通过与钙离子结合实现其生物学功能,调控细胞信号传导和细胞生命周期等过程,且钙结......
用RT-PCR方法扩增并克隆了三种人外周型苯二氮卓受体PBRcDNA,测序表明,442bp片段与文献报道相比缺失84bp编码序列,其转录水平高于正常PBR,该序列编码一个与PBR结构相......
以鲑鱼基因组DNA为模板,采用PCR获得sCT基因,并为DNA序列分析所证实。以pGEX-3X为表达载体,利用体外定点突变技术成功地构建了融合蛋白GST-sCT的重组表达质粒pGEX-3X-sCT,在大......
为了解马尾松(Pinus massoniana)磷酸甘油酸激酶1(PGK1)与胞质溶胶葡萄糖磷酸异构酶(GPIC)的功能,采用RACE技术克隆了PmPGK1和PmGP......
目的 构建辐射敏感启动子Egr-1诱导人线粒体促凋亡基因(Smac)表达的腺病毒穿梭载体pshuttle-Egr1-hSmac.方法 利用RT-PCR方法从5月......
Induction of Chondrogenesis of Adipose-derived Stem Cells by Novel Recombinant TGF-β3 Fusion Protein
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目的 构建rAAV2-hGAD65重组载体,观察其体内外功能.方法 应用RT-PCR的方法克隆hGAD65基因,与AAV载体连接得到重组载体(rAAV2-hGAD6......
为开展禾谷缢管蚜(Rhopalosiphum padi Linnaeus)抗性的分子生物学研究,设计简并引物并采用RT—PCR技术,克隆了长度分别为260、331、33......
目的构建肿瘤坏死因子α诱导蛋白3相互作用蛋白1(TNIP1)真核表达载体(p EGFP-N3-TNIP1),观察了解外源TNIP1蛋白的细胞内定位及其在He L......
以人APKD基因探针218Ep6在大鼠基因组文库中筛选到阳性克隆pM1,经充分鉴定证实含有探针的同源顺序。......
为了获得有效的保护性抗原分子,我们对已构建的日本血吸虫成虫cDNA库的免疫筛选中所获得的阳性克隆λSj514的cDNA插入片段进行PCR扩增,扩增产物亚克隆......
主要介绍近年来高等植物中生物活性GAs的生物合成,拟南芥GA生物合成途径中关键酶基因(GA1_GA5)的克隆和GA3基因CYP701A3在酵母(Sacchar......
以质粒PBR322为载体,BamHI部分酶切片段为目的焉段成功构建了大鼠基因组文库并进行了鉴定,文库总重组子数为3.2×10^7,插入比例95%,为大鼠APKD基因的克隆筛选......